Filtration of Nematodes using an Integrated Microcapillary System

TitleFiltration of Nematodes using an Integrated Microcapillary System
Publication TypeJournal Article
Year of Publication2014
AuthorsLaki AJ, Iván K, Fok É, Civera P
ISSN2191-1630, 2191-1649
KeywordsBiomaterials, Biophysics and Biological Physics, Blood-borne parasitosis, Circuits and Systems, Microfluidic filtration device, Nanotechnology, Veterinarian diagnostics

We demonstrate a novel microfluidic device to detect living parasites from native blood using monolithic polydimethylsiloxane (PDMS) structures. Several blood-borne and tick-borne parasites such as dirofilariosis can be investigated by this microcapillary system. Inside this microfluidic device, a special flow-through separator structure has been implemented which contains a cylindrical active zone, where the microfilariae or other few micron-size pathogens remain trapped. The center region is partially surrounded by microcapillaries which are positioned along an isobaric line to aid in filtration efficiency through pressure equalization. The developed test can be optimized for a specific nematode or pathogen detection by adjusting the capillary width.